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| Catalog Number | orb779526 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen(Plg). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 15.63-1000 ng/mL |
| Assay Time | 3.5h |
| Concentration | 1000 ng/mL |
| UniProt ID | Q01177 |
| Sensitivity | 6.8 ng/mL |
| Alternative names | PL ELISA Kit, Plasmin ELISA Kit, Activation peptid Read more... |
| Note | For research use only |
| Application notes | standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Plg in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
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Rat PLG ELISA Kit [orb339758]
Rat
62.5 ng/mL-4000 ng/mL
15.6 ng/mL
24 T, 48 T, 10 x 96 T, 5 x 96 T, 96 T
