Rat Plasminogen (Plg) ELISA Kit

SKU: orb779526

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen(Plg).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Plg in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 6.8 ng/mL
• Concentration: 1000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PL ELISA Kit, Plasmin ELISA Kit, Activation peptide ELISA Kit, Angiostatin ELISA Kit

Quick Database Links

UniProt

Q01177