Rat Phosphotylinosital 3 kinase (PI3K) ELISA Kit

SKU: orb782226

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI3K(Phosphotylinosital 3 kinase).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PI3K. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PI3K. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PI3K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PI3K in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 3.9 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PI3K ELISA Kit, Phosphotylinosital 3 kinase ELISA Kit, ELISA Kit

References

Raghda N El Mahdy 1, Manar A Nader 1, Manar G Helal 1, Sally E Abu-Risha 2, Marwa E Abdelmageed Tiron ameliorates acetic acid-induced colitis in rats: Role of TGF-β/EGFR/PI3K/NF-κB signaling pathway Int Immunopharmacol ., (2024)

PubMed: 38286073

Applications

ELISA

Reactivity

Rat

AM Alnakhli, A Saleh, AM Kabel, RS Estfanous Perindopril Ameliorates Sodium Valproate-Induced Rat Model of Autism: Involvement of Sirtuin-1, JAK2/STAT3 Axis, PI3K/Akt/GSK-3β Pathway, and PPAR Medicina, (2024)

PubMed:

Applications

ELISA

Reactivity

Rat