Rat phosphorylated microtubule-Associated Protein tau (pMAPT /pTAU) ELISA Kit

• Catalog Number: orb1088242
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to phosphorylated microtubule-associated protein tau(pMAPT /pTAU). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to phosphorylated microtubule-associated protein tau(pMAPT /pTAU). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain phosphorylated microtubule-associated protein tau(pMAPT /pTAU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of phosphorylated microtubule-associated protein tau(pMAPT /pTAU) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Concentration: 2000 pg/mL
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.37pg/mL
• Sample Types: serum, plasma, other biological fluids
• Alternative names: pMAPT /pTAU
• Note: For research use only