Rat Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

SKU: orb780597

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IIA(PLA2G2A).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 12 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PLA2G2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PLA2G2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PLA2G2A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.19-12 ng/mL
• Sensitivity: 0.082 ng/mL
• Concentration: 12 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PLA2 ELISA Kit, MOM1 ELISA Kit, PLA2B ELISA Kit, PLA2L ELISA Kit, PLA2S ELISA Kit, PLAS1 ELISA Kit, sPLA2(platelets,synovial fluid) ELISA Kit, Non-pancreatic secretory phospholipase A2 ELISA Kit, Phosphatidylcholine 2-acylhydrolase 2A ELISA Kit

Quick Database Links

UniProt

P14423