Rat Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit

SKU: orb1736455

Description

The Rat Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit uses a sandwich enzyme immunoassay to quantify Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) in rat tissue homogenates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.073 ng/mL.

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIK3C2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIK3C2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIK3C2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIK3C2a in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.073 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Quick Database Links

UniProt

A0A0G2K344