Rat ovalbumin Specific IgE (OVA sIgE) ELISA Kit

• Catalog Number: orb866962
• Category: Assays and Kits
• Description: Sandwich Rat OVA sIgE ELISA Kit
• Reactivity: Rat
• Concentration: 20 µg/mL
• Tested applications: ELISA
• Application notes: standard: 20 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OVA sIgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OVA sIgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OVA sIgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OVA sIgE in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.312-20ug/ml
• Sensitivity: 0.125ug/ml
• Sample Types: Serum, plasma
• Alternative names: OVA SIgE ELISA Kit
• Research Area: Hematology, Immunology & Inflammation, Infectious
• Note: For research use only
• Expiration Date: Please enquire.