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| Catalog Number | orb782729 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Orexin(OX) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Orexin(OX). |
| Reactivity | Rat |
| Concentration | 1000 pg/mL |
| UniProt ID | O55232 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OX in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.43 pg/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | HCRT ELISA Kit, PPOX ELISA Kit, Orexin A ELISA Kit Read more... |
| Note | For research use only |
Rat Orexin A ELISA Kit [orb410701]
Rat
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T- Item 1 of 1
