Rat OPN ELISA Kit Discontinued

SKU: orb775404

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin(OPN).

Research Area

Cancer Biology, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 20 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OPN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.128 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

SPP1 ELISA Kit, BNSP ELISA Kit, BSPI ELISA Kit, ETA1 ELISA Kit, Secreted Phosphoprotein 1 ELISA Kit, Bone Sialoprotein I ELISA Kit, Early T-Lymphocyte Activation 1 ELISA Kit, Nephropontin ELISA Kit, Urinary stone protein ELISA Kit, Uropontin ELISA Kit

Quick Database Links

UniProt

P08721

Xinyue Yang 1, Lijun Wu 2, Cheng Li 1, Shujun Li 3, Wentao Hou 3, Yulin Hao 3, Yiping Lu 2, Lei Li 1 Synergistic Amelioration of Osseointegration and Osteoimmunomodulation with a Microarc Oxidation-Treated Three-Dimensionally Printed Ti-24Nb-4Zr-8Sn Scaffold via Surface Activity and Low Elastic Modulus ACS Appl Mater Interfaces, (2024)

PubMed: 38205810

Applications

ELISA

Reactivity

Rat