Rat Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit

SKU: orb781065

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3(NCOA3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NCOA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NCOA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NCOA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NCOA3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.114 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

RAC3 ELISA Kit, ACTR ELISA Kit, AIB1 ELISA Kit, CAGH16 ELISA Kit, CTG26 ELISA Kit, SRC3 ELISA Kit, TNRC14 ELISA Kit, TNRC16 ELISA Kit, TRAM1 ELISA Kit, pCIP ELISA Kit, Amplified In Breast 1 ELISA Kit, Steroid Receptor Coactivator-3 ELISA Kit, Thyroid Hormone Receptor Activator Molecule 1 ELISA Kit

Quick Database Links

UniProt

Q9EPU2