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Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

Catalog Number: orb1806928

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1806928
CategoryAssays and Kits
DescriptionRat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit
TargetNGAL
ReactivityRat
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NGAL. You can calculate the concentration of Rat NGAL in the samples by comparing the OD of the samples to the standard curve.
Assay Time3.5H
Range62.5-4000pg/mL
Sensitivity37.5pg/mL
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Alternative namesLCN2, Lipocalin 2, Oncogene 24p3, MSFI
NoteFor research use only
Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit