Rat Neutral Sphingomyelinase (NSMASE) ELISA Kit

SKU: orb780034

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutral Sphingomyelinase(NSMASE).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NSMASE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NSMASE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NSMASE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NSMASE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.065 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SMPD2 ELISA Kit, N-SMase ELISA Kit, ISC1 ELISA Kit, Neutral Sphingomyelin Phosphodiesterase ELISA Kit, Sphingomyelin Phosphodiesterase 2,Neutral Membrane ELISA Kit, Lyso-platelet-activating factor-phospholipase C ELISA Kit

Quick Database Links

UniProt

Q9ET64