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Rat Neurotensin (NT) ELISA Kit

Catalog Number: orb779912

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb779912
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurotensin(NT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurotensin(NT).
Tested applicationsELISA
ReactivityRat
Range46.88-3000 pg/mL
Assay Time2.5h
Concentration3000 pg/mL
UniProt IDP20068
Sensitivity14.5 pg/mL
Alternative namesNTS ELISA Kit, NTS1 ELISA Kit, NmN-125 ELISA Kit,
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NoteFor research use only
Application notesstandard: 3000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Expiration DatePlease enquire.
Rat Neurotensin (NT) ELISA Kit