Rat Neuropeptide S (NPS) ELISA Kit

• Catalog Number: orb775520
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neuropeptide S(NPS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropeptide S(NPS).
• Reactivity: Rat
• Concentration: 1000 pg/mL
• UniProt ID: P0C0P7
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPS in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.92 pg/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: NP-S ELISA Kit
• Research Area: Metabolism Research, Neuroscience
• Note: For research use only
• Expiration Date: Please enquire.

References

Harish M Kawade 1, Utkarsh P Patil 2, Deepali M Pandhare 3, Nishikant K Subhedar 4, Dadasaheb M Kokare Neuropeptide S system mediates nicotine-induced reward-facilitatory behavior Neuropharmacology ., (2025)

PubMed: 40466934