Rat Neuraminidase (NEU) ELISA Kit

• Catalog Number: orb1173593
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuraminidase(NEU). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuraminidase(NEU). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuraminidase(NEU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuraminidase(NEU) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Concentration: 10 ng/mL
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NEU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NEU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NEU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NEU in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: Acetylneuraminyl hydrolase, exo-alpha-sialidase, G
• Research Area: Metabolism Research, Neuroscience, Signal Transduc
• Note: For research use only