Rat Nerve Growth Factor IB (NGFIB) ELISA Kit

SKU: orb780138

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor IB(NGFIB).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NGFIB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NGFIB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NGFIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NGFIB in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GFRP1 ELISA Kit, HMR ELISA Kit, N10 ELISA Kit, NAK-1 ELISA Kit, NGFIB ELISA Kit, NP10 ELISA Kit, NUR77 ELISA Kit, TR3 ELISA Kit, DHR38 ELISA Kit, TIS1 ELISA Kit, Orphan nuclear receptor HMR ELISA Kit, Testicular receptor 3 ELISA Kit, Nuclear receptor subfamily 4 group A member 1 ELISA Kit

Quick Database Links

UniProt

P22829