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| Catalog Number | orb780694 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin(NEP). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 78.13-5000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 5000 pg/mL |
| UniProt ID | P07861 |
| Sensitivity | 31 pg/mL |
| Alternative names | CD10 ELISA Kit, MME ELISA Kit, CALLA ELISA Kit, SF Read more... |
| Note | For research use only |
| Application notes | standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NEP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
Rat MME ELISA Kit [orb410414]
Rat
47 pg/mL-3000 pg/mL
11.75 pg/mL
10 x 96 T, 48 T, 5 x 96 T, 96 T, 24 T
