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| Catalog Number | orb780483 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 7, Cardiac Muscle, Beta(MYH7). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 125-8000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 8000 pg/mL |
| UniProt ID | P02564 |
| Sensitivity | 53 pg/mL |
| Alternative names | CMD1S ELISA Kit, CMH1 ELISA Kit, MPD1 ELISA Kit, M Read more... |
| Note | For research use only |
| Application notes | standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH7 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
