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Rat Multimerin 1 (MMRN1) ELISA Kit

SKU: orb780570
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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multimerin 1(MMRN1).

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMRN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMRN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMRN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMRN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityRat
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range78.13-5000 pg/mL
Sensitivity35 pg/mL
Concentration5000 pg/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
DisclaimerFor research use only

Alternative Names

ECM ELISA Kit, EMILIN4 ELISA Kit, GPIa ELISA Kit, MMRN ELISA Kit, Glycoprotein Ia ELISA Kit, Elastin microfibril interface located protein 4 ELISA Kit, Endothelial cell multimerin ELISA Kit, Platelet glycoprotein Ia ELISA Kit, 155 kDa platelet multimerin ELISA Kit

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Rat Multimerin 1 (MMRN1) ELISA Kit

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Rat Multimerin 1 (MMRN1) ELISA Kit (orb780570)

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48 T
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