Rat Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit

SKU: orb782694

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.067 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LC3B ELISA Kit, MAP1LC3B ELISA Kit, MAP1ALC3 ELISA Kit, Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit, Autophagy-related protein LC3 B ELISA Kit, Autophagy-related ubiquitin-like modifier LC3 B ELISA Kit, MAP1 light chain 3-like protein 2 ELISA Kit, MAP1A/MAP ELISA Kit

Quick Database Links

UniProt

Q62625