Rat Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) ELISA Kit

• Catalog Number: orb1146964
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan(MCSP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan(MCSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Associated Chondroitin Sulfate Proteoglycan(MCSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Associated Chondroitin Sulfate Proteoglycan(MCSP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Concentration: 10 ng/mL
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MCSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MCSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MCSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MCSP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Alternative names: CSPG4, MSK16, HMW-MAA, MCSP, MCSPG, MEL-CSPG, NG2,
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: Please enquire.