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Rat Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit

Catalog Number: orb780452

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb780452
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase Like Protein 2(LOXL2).
ReactivityRat
Concentration10 ng/mL
UniProt IDB5DF27
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LOXL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LOXL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LOXL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LOXL2 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.054 ng/mL
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Alternative namesLOR2 ELISA Kit, WS9-14 ELISA Kit, Lysyl oxidase ho
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NoteFor research use only
Rat Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit