Rat Lysozyme (LZM) ELISA Kit

• Catalog Number: orb2667162
• Category: Assays and Kits
• Description: Rat Lysozyme (LZM) ELISA Kit
• Application notes: standard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LZM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LZM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LZM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LZM in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 µg/mL
• Sensitivity: 0.053 µg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: LYZ; Renal Amyloidosis; N-Acetylmuramide Glycanhyd
• Note: For research use only