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Rat Lipase, Lipoprotein (LIPD) ELISA Kit

Catalog Number: orb775447

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775447
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Lipoprotein(LIPD).
Tested applicationsELISA
ReactivityRat
Range1.57-100 ng/mL
Assay Time3.5h
Concentration100 ng/mL
UniProt IDQ06000
Sensitivity0.62 ng/mL
Alternative namesLPL ELISA Kit, Lipoprotein Lipase ELISA Kit
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LIPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LIPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LIPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LIPD in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Rat Lipase, Lipoprotein (LIPD) ELISA Kit