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| Catalog Number | orb775561 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin(LTF). |
| Reactivity | Rat |
| Concentration | 400 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LTF in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 6.25-400 ng/mL |
| Sensitivity | 2.9 ng/mL |
| Sample Types | Serum, plasma and other biological fluids. |
| Alternative names | HLF2 ELISA Kit, LF ELISA Kit, GIG12 ELISA Kit, Lac Read more... |
| Note | For research use only |
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