Rat Islet Amyloid Polypeptide (IAPP) ELISA Kit

SKU: orb775466

Description

The Rat Islet Amyloid Polypeptide (IAPP) ELISA Kit is designed to quantify Islet Amyloid Polypeptide (IAPP) in rat serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 7.82-500 pg/mL and a sensitivity of 2.73 pg/mL.

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.73 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

IAP ELISA Kit, DAP ELISA Kit, Amylin ELISA Kit, Insulinoma Amyloid Polypeptide ELISA Kit, Diabetes-associated peptide ELISA Kit

Quick Database Links

Gene Symbol

IAPP

UniProt

P12969