Rat Interleukin 18 Binding Protein (IL18BP) ELISA Kit

• Catalog Number: orb780661
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Binding Protein(IL18BP).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 0.63-40 ng/mL
• Assay Time: 3.5h
• Concentration: 40 ng/mL
• Sensitivity: 0.249 ng/mL
• Alternative names: IL18BPa ELISA Kit, MC51L-53L-54L Homolog Gene Prod
• Note: For research use only
• Application notes: standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL18BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL18BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL18BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL18BP in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.