Rat Interleukin 1 Beta (IL1b) ELISA Kit

SKU: orb775137

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta(IL1b).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.9 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IL1-B ELISA Kit, IL1-Beta ELISA Kit, IL1F2 ELISA Kit, IL-1β ELISA Kit, Interleukin-1 Family Member 2 ELISA Kit, Catabolin ELISA Kit

Quick Database Links

UniProt

Q63264

References

Z Sun, HY Ren, JY Song Tong-luo Qu-tong Plaster relieves chronic muscle pain by reducing inflammation via NCF1-CXCL10-ICAM1 axis in SD rats Tradit Med Res, (2025)

PubMed:

Applications

ELISA

Reactivity

Rat