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| Catalog Number | orb776427 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Parathyroid Hormone(PTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Parathyroid Hormone(PTH). |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 12.5-800 pg/mL |
| Assay Time | 3.5h |
| Concentration | 800 pg/mL |
| Sensitivity | 3.76 pg/mL |
| Alternative names | iPTH ELISA Kit, Intact Parathyroid Hormone ELISA K Read more... |
| Note | For research use only |
| Application notes | standard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
