Rat Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit

• Catalog Number: orb2667228
• Category: Assays and Kits
• Description: Rat Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
• UniProt ID: P15473
• Application notes: standard: 20 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.312-20 µg/mL
• Sensitivity: 0.133 µg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: BP53; IBP3; Growth Hormone-Dependent Binding Prote
• Note: For research use only