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Rat Inositol Triphosphate (IP3) ELISA Kit

Catalog Number: orb1736566

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1736566
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityRat
Concentration1000 pg/mL
Application notesstandard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IP3 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity5.73 pg/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesInsP3; Inositol 1,4,5-Trisphosphate; Triphosphoino
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NoteFor research use only
Rat Inositol Triphosphate (IP3) ELISA Kit