Rat Immunoglobulin M (IgM) ELISA Kit

• Catalog Number: orb2668053
• Category: Assays and Kits
• Description: Rat Immunoglobulin M (IgM) ELISA Kit
• Application notes: standard: 200 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgM in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 µg/mL
• Sensitivity: 1.35 µg/mL
• Sample Types: serum and plasma
• Alternative names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chai
• Research Area: Hematology, Immunology & Inflammation, Infectious
• Note: For research use only
• Expiration Date: Please enquire.