Rat Immunoglobulin E (IgE) ELISA Kit

SKU: orb776458

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E(IgE).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 ng/mL
• Sensitivity: 14.4 ng/mL
• Concentration: 2000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGHE ELISA Kit, Immunoglobulin Heavy Constant Epsilon ELISA Kit, Ig epsilon chain C region ELISA Kit