Rat IL-2 R alpha / CD25 Protein, His Tag (MALS verified)

SKU: orb3158261

Description

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.3 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: IL-2 R alpha
• Reactivity: Rat
• Tag: C-10×His
• Expression Region: Glu 22 - Gln 235
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 26.3 kDa
• Purity: 90%

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

IL-2 R alpha

RefSeq:P26897