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Catalog Number | orb754424 |
---|---|
Category | Proteins |
Description | Recombinant rat IL-10 protein |
Tag | N-terminal His Tag |
Reactivity | Rat |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
Purity | Greater than 95% as determined by SDS-PAGE |
UniProt ID | P29456 |
MW | 17.5 kDa |
Tested applications | ELISA, WB |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Source | E.Coli |
Expression Region | 19-178 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Alternative names | CSIF, Cytokine synthesis inhibitory factor, GVHDS, Read more... |
Note | For research use only |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses. | |
16.6 kDa |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97 % by SDS-PAGE and HPLC analyses | |
16.6 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
16 kDa | |
E.Coli |
> 80%, determined by SDS-PAGE | |
This protein contains the rat IL10 (NP_036986.2) (Ser19-Asn178) was expressed and purified with an initial Met. |
> 95% as analyzed by SDS-PAGE and HPLC. | |
8-22 kDa, observed by reducing SDS-PAGE. | |
CHO |