Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

• Catalog Number: orb780880
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha(HIF2a).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 78.13-5000 pg/mL
• Assay Time: 3.5h
• Concentration: 5000 pg/mL
• UniProt ID: Q9JHS1
• Sensitivity: 31 pg/mL
• Alternative names: EPAS1 ELISA Kit, HLF ELISA Kit, MOP2 ELISA Kit, PA
• Note: For research use only
• Application notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HIF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HIF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HIF2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HIF2a in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.