Rat Hypoxia Inducible Factor 1 Alpha (HIF-1α) ELISA Kit

SKU: orb1807000

Description

Rat Hypoxia Inducible Factor 1 Alpha (HIF-1α) ELISA Kit

Research Area

Protein Biochemistry

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenate, Cell lysate
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 7.81-500pg/mL
• Sensitivity: 4.69 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

Quick Database Links

Gene Symbol

HIF1a

UniProt

O35800