Rat Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

• Catalog Number: orb778470
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule(HCAM).
• Reactivity: Rat
• Concentration: 10 ng/mL
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HCAM in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.063 ng/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CDW44 ELISA Kit, CD44 ELISA Kit, CSPG8 ELISA Kit,
• Note: For research use only