Rat HMGB-1(High Mobility Group Protein B1) Microsample ELISA Kit

• Catalog Number: orb2999411
• Category: Assays and Kits
• Description: Rat HMGB-1(High Mobility Group Protein B1) Microsample ELISA Kit
• UniProt ID: P63159
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.067 ng/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Bi
• Note: For research use only