Rat High Affinity Choline Transporter (ChT) ELISA Kit

SKU: orb781070

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter(ChT).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ChT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ChT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ChT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ChT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 29 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

SLC5A7 ELISA Kit, hCHT, CHT1 ELISA Kit, Solute Carrier Family 5 Member 7,Choline Transporter ELISA Kit, Hemicholinium-3-sensitive choline transporter ELISA Kit