Rat Hexosaminidase B Beta (HEXb) ELISA Kit

SKU: orb775582

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexosaminidase B Beta(HEXb).

Research Area

Cancer Biology, Metabolism Research, Neuroscience, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HEXb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HEXb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HEXb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HEXb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.56 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Hex-B ELISA Kit, ENC-1AS ELISA Kit, HCC-7 ELISA Kit, Beta-N-acetylhexosaminidase subunit beta ELISA Kit, Cervical cancer proto-oncogene 7 protein ELISA Kit, N-acetyl-beta-glucosaminidase subunit beta ELISA Kit

Quick Database Links

UniProt

Q6AXR4