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| Catalog Number | orb2667431 |
|---|---|
| Category | Assays and Kits |
| Description | Rat Heat Shock Protein HSP 90-Alpha (HSP90α) ELISA Kit |
| Range | 1.56-100 ng/mL |
| Assay Time | 3.5h |
| UniProt ID | P82995 |
| Sensitivity | 0.51 ng/mL |
| Alternative names | heat shock protein 90 alpha family class A member Read more... |
| Note | For research use only |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSP90α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSP90α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSP90α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSP90α in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates |
| Expiration Date | Please enquire. |
