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| Catalog Number | orb2817077 |
|---|---|
| Category | Assays and Kits |
| Description | Rat HCy(Homocysteine) ELISA Kit |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HCy. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HCy in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 2h |
| Range | 125-8000 ng/mL |
| Sensitivity | 39.99 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | Homocysteine |
| Note | For research use only |
