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Rat Growth Regulated Oncogene Alpha (GROα/CXCL1) Microsample ELISA Kit

Catalog Number: orb1806736

DispatchUsually dispatched within 5-10 working days
$ 330.00
Catalog Numberorb1806736
CategoryAssays and Kits
DescriptionRat Growth Regulated Oncogene Alpha (GROα/CXCL1) Microsample ELISA Kit
ReactivityRat
Range15.63-1000pg/mL
Assay Time3H
TargetGROα/CXCL1
Sensitivity9.38 pg/mL
Alternative namesNAP3, GRO1, GRO-A, MGSA, MGSA-A, SCYB1, FSP, CINC-
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GROα/CXCL1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat GROα/CXCL1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat GROα/CXCL1. You can calculate the concentration of Rat GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma and Tissue homogenate and other biological samples;Sample volume:25μL
Expiration DatePlease enquire.
Rat Growth Regulated Oncogene Alpha (GROα/CXCL1) Microsample ELISA Kit