Rat Growth Hormone (GH) ELISA Kit

SKU: orb781720

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone(GH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GH1 ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, hGH-N ELISA Kit, Somatotropin ELISA Kit, Hygetropin ELISA Kit, Jintropin ELISA Kit, Kigtropin ELISA Kit, Pituitary Growth Hormone ELISA Kit, Growth Hormone, Normal ELISA Kit

Quick Database Links

UniProt

P01244