Rat Growth Factor, Augmenter of Liver Regeneration (GFER) ELISA Kit

SKU: orb1146767

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor, Augmenter Of Liver Regeneration(GFER). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Growth Factor, Augmenter Of Liver Regeneration(GFER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor, Augmenter Of Liver Regeneration(GFER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Factor, Augmenter Of Liver Regeneration(GFER) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GFER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GFER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GFER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GFER in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 29 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ERV1, ALR, HERV1, HPO, HPO1, HPO2, HSS, FAD-linked sulfhydryl oxidase ALR, Augmenter of liver regeneration

Quick Database Links

UniProt

Q63042