Rat Growth Differentiation Factor 3 (GDF3) ELISA Kit

SKU: orb780125

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 3(GDF3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 29.5 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Vgr2 ELISA Kit, Vg-Related Gene 2 ELISA Kit