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Rat Glycoprotein 130 (gp130) ELISA Kit

Catalog Number: orb776360

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776360
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 130(gp130).
Tested applicationsELISA
ReactivityRat
Range0.63-40 ng/mL
Assay Time3.5h
Concentration40 ng/mL
Sensitivity0.254 ng/mL
Alternative namesCDw130 ELISA Kit, CD130 ELISA Kit, IL6ST ELISA Kit
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NoteFor research use only
Application notesstandard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat gp130. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat gp130. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat gp130, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat gp130 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Rat Glycoprotein 130 (gp130) ELISA Kit

  • Rat CD130 ELISA Kit [orb1473326]

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    780 pg/ml - 50000 pg/ml

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    0.78 ng/ml - 50 ng/ml

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