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| Catalog Number | orb775579 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 1(GSTa1). |
| Reactivity | Rat |
| Concentration | 20 ng/mL |
| UniProt ID | P00502 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GSTa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GSTa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GSTa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GSTa1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.129 ng/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | GSTA1 ELISA Kit, GST2 ELISA Kit, GSTA1-1 ELISA Kit Read more... |
| Note | For research use only |
- Item 1 of 1
Rat α-GST (α-Glutathione S-Transferases) Quick ELISA Kit [orb2567531]
Rat
37.5-2400pg/ml
22.5pg/ml
96 T, 48 T 
Rat Gsta1 ELISA Kit [orb410680]
Rat
0.156 mU/mL-10 mU/mL
0.039 mU/mL
24 T, 10 x 96 T, 96 T, 5 x 96 T, 48 T
