Rat Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit

SKU: orb779830

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 3, Plasma(GPX3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GPX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.36 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GSHPX3 ELISA Kit, GPx-P ELISA Kit, GSHPx-P ELISA Kit, GPXP ELISA Kit, Plasma glutathione peroxidase ELISA Kit, Extracellular glutathione peroxidase ELISA Kit

Quick Database Links

UniProt

P23764