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Rat Glucoprotein 130 (gp130) ELISA Kit

Catalog Number: orb1946993

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1946993
CategoryAssays and Kits
DescriptionRat Glucoprotein 130 (gp130) ELISA Kit
ReactivityRat
Range0.16-10ng/mL
Assay Time3.5H
Targetgp130
UniProt IDP40190
Sensitivity0.1ng/mL
Alternative namesIL6ST, CD130, CDW130, IL-6RB, IR6RB, Interleukin 6
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat gp130. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat gp130, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat gp130. You can calculate the concentration of Rat gp130 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Rat Glucoprotein 130 (gp130) ELISA Kit